^{2nd Middle East Pharmacy and Pharmaceutical Conference}

Benzene Sulphonamide is an important pharmacophore in modern drug discovery and are one of the least expensive chemotherapeutic agents. In order to evaluate the synthesized compounds as COX-2 inhibitors a series of compounds with definite IC50 values were selected as the dataset by using Padel. The obtained IC50 values are then converted into corresponding pIC50. Among the compounds selected 75% were utilized as a training set and remaining 25% were chosen as a test subset for validating the QSAR model. The model is extensively validated according to OECD standards, so that its robustness, stability, low correlation of descriptors and good predictive power are proven. In addition, it is found that the model fit is not the product of a random correlation. Based on the model obtained the series of designed compounds were synthesized. The structures of the synthesized compounds were elucidated by IR spectra and were then evaluated for their anti-inflammatory activity using carrageenan- induced paw edema method against the standard Diclofenac. In silico studies were carried out to define the interaction between the synthesized compounds with COX-2 enzyme. Results from in vivo and in vitro studies of synthesized compounds were found to be in good correlation with in silico study.